Cre-loxP system
| Method for the introduction of genetic modifications into specific genes by homologous recombination using Cre a site-specific, bacteriophage P1-derived recombinase.
The Cre recombinase cuts at the loxP-tagged genes.
Source | Bacteriophage P1 protein overexpressed in Escherichia coli. DNA synthetic |
Oligomer state | 2:1 Complex of protein to DNA |
Mr | 38 500 × 2 |
Amino acids | 343 × 2 |
Nucleotides | 16 d5'(TATAACTTCGTATAGC) and 19 d5'(ATATGCTATACGAAGTTAT) annealed together to give the loxA cognate DNA |
|