Restriction landmark genomic scanning (RLGS) Techniques developed for analyzing genomic DNA (fingerprinting) of higher organisms. It is based on the principle that restriction enzymes cutting sites can be uses as landmarks. Genomic DNA is directly labelled at the restiriction sites and two-dimensionall electrophoresis is employed to resolve and identify these landmarks. The procedure gives a 2-D pattern with thousands of scattered spots of landmarks after autoradiography.